Journal: Journal of visualized experiments : JoVE

Article Title: Drosophila as a model to study wound-induced polyploidization

doi: 10.3791/61252

Figure Lengend Snippet: List of all reagents and equipment required for this study.

Article Snippet: Protocol to make antibody can be found (Ref. # 4 and 8 ) Rabbit anti-RFP Primary Antibody MBL PM005 For immunostaining mCD8-RFP fly epithelium Mouse anti-Fasciclin III (Drosophila) Primary Antibody Developmental Studies Hybridoma Bank 7G10 For immunostaining epithelial cell-cell junctions Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate Thermofisher A11001 For secondary immuostaining Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 568 conjugate Thermofisher A11031 For secondary immuostaining Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate Thermofisher A21206 For secondary immuostaining Donkey anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 568 conjugate Thermofisher A10042 For secondary immuostaining Triton X-100 Sigma 10789704001 For immuostaining Bovine Serum Albumin, 30% Sigma A7284-500ML For immuostaining Click-iT EdU 594 Kit Thermofisher C10339 For EdU assay DAPI Sigma D9542-10MG For immuostaining Glass slides Fisher Scientific 12-518-104C For mounting Coverslips Thermofisher 3406 For mounting Mouting media Vector Laboratories H-1000 Anti-fade mounting media to prevent photo bleaching during imaging Nail polish Electron Microscopy Sciences 72180 For sealing slides 50mL Conical Centrifuge Tubes Fisher Scientific 14-432-22 For preparing staining reagents in Ortibal shaker Fisher Scientific 02-217-988 For immuostaining Imaging and Analysis AxioImager M2 with Apotome Zeiss NA For imaging samples Fiji/ ImageJ (image analysis software) NIH https://imagej.nih.gov/ij For image analysis Excel Microsoft For performing ploidy calculations D. melanogaster strains epi-Gal4 Bloomington Drosophila Stock Center (b) b38793 Losick et al. Current Biology, 2013 epi-Gal4, UAS-mCD8.RFP Bloomington Drosophila Stock Center (b) b38793, b27392 Losick et al. Current Biology, 2013 UAS-myc RNAi Bloomington Drosophila Stock Center (b) b36123 Grendler et al. Development, 2019 UAS-Myc Bloomington Drosophila Stock Center (b) b9674 Grendler et al. Development, 2019 UAS-fzr RNAi, UAS-Stg VDRC (v) and Bloomington Drosophila Stock Center (b) v25550, b56562 Grendler et al. Development, 2019 UAS-E2F RNAi, UAS-RacDN VDRC (v) and Bloomington Drosophila Stock Center (b) v108837, b6292 Losick et al. Current Biology, 2013 Open in a separate window List of all reagents and equipment required for this study.

Techniques: Dissection, Staining, Saline, Immunostaining, EdU Assay, Plasmid Preparation, Imaging, Electron Microscopy, Software

Journal: Hippocampus

Article Title: Adult-born granule cell mossy fibers preferentially target parvalbumin-positive interneurons surrounded by perineuronal nets

doi: 10.1002/hipo.23296

Figure Lengend Snippet: Reagent resource table

Article Snippet: Sections from all other experiments were imaged with a Zeiss confocal microscope LSM 700 (Oberkochen, Germany). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Name Host species Dilution Type Company Catalog # RRID # Parvalbumin (PV) Mouse 1:500 Primary Sigma-Aldrich (St. Louis, MO) P3088 AB_477329 Parvalbumin (PV) Rabbit 1:2000 Primary Abcam (Cambridge, UK) ab11427 AB_298032 GFP Rabbit 1:500 Primary Molecular Probes (Eugene, OR) {"type":"entrez-nucleotide","attrs":{"text":"G10362","term_id":"942211","term_text":"G10362"}} G10362 AB_2536526 Wisteria floribunda agglutinin (WFA) lectin N/A 1:500 Primary Sigma-Aldrich L1516 AB_2620171 3RTau Mouse 1:500 Primary Millipore (Burlington, MA) 05–803 AB_310013 Synaptophysin (SYP) Mouse 1:200 Primary Millipore MAB329 AB_94786 PSA-NCAM Rat 1:400 Primary BD Pharmingen (San Diego, CA) 556325 AB_396363 Donkey anti-mouse 568 Donkey 1:500 Secondary Invitrogen (Carlsbad, CA) A10037 AB_2534013 Donkey anti-rat 488 Donkey 1:500 Secondary Abcam ab150153 AB_2737355 Donkey anti-rabbit 488 Donkey 1:500 Secondary Invitrogen A21206 AB_2535792 Donkey anti-rabbit 568 Donkey 1:500 Secondary Invitrogen A10042 AB_2534017 Anti-streptavidin 647 N/A 1:500 Secondary Molecular Probes {"type":"entrez-protein","attrs":{"text":"S21374","term_id":"99986","term_text":"pir||S21374"}} S21374 AB_2336066 Anti-streptavidin 488 N/A 1:500 Secondary Molecular Probes S11223A AB_2315383 Open in a separate window Reagent resource table 2.5 |.

Techniques: